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NGS Sequencing Helper - High-throughput Fluorometer Fluo-800
ALLSHENG Release time:2023-04-12 ALLSHENG Source: ALLSHENG Pageviews:3133

"During NGS sequencing, accurate library concentration is a precondition for obtaining high quality sequencing data. If the sample concentration is too low, it may result in low data output and thus not sufficient sequencing data. If the sample concentration is too high, it will produce low-quality sequencing data and may even lead to sequencing failure. Therefore, accurate and reliable library concentration plays an important role in the sequencing process."

Fluo-800 fluorometer provides rapid fluorescence detection of eight samples simultaneously, with high precision detection method to effectively reduce the variability of library sample assays. The intuitive user interface and optimized software design are more user-friendly to fit the researcher's usage habits and greatly reduce the sequencing preparation time.

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New Product Features

1 Simple and intuitive

●7 inch color touch screen

2 Fast detection

●Quickly and accurately detect DNA, RNA, and proteins in 6 seconds

3 Accurate quantification

●High accuracy with only 1-20 μL samples

4 Multiple software functions

●Reagent concentration calculator, molarity calculator, concentration normalization

5 Flexible data export

●Up to 20000 programs can be stored; can be exported directly to U disk, or connected to an external printer

6 Data save

●Save sample data as a CSV or PDF file

7 Open reagent application

●Can match reagents from different manufacturers


Performance Comparison Between Fluo-800 and QF


When measuring high concentration DNA samples, the deviation between Fluo-800 and QF is within 10%.

Table 1 Comparison of Test Data Provided by Customers

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The 8-well CV values of Fluo-800 and QF were similar when measuring the same ultra-low concentration cfDNA sample.

Table 2 Comparison of Inter-well Differences For The Same Concentration of cfDNA

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The deviation values between Fluo-800 and QF were all within 10% when measuring cfDNA samples of different concentrations (except for well 8).

Table 3 Comparison of D-value in cfDNA at Different Concentrations

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Allsheng high-throughput fluorometer Fluo-800 is specifically designed for NGS, providing you with twice the work with half the effort during multi sample sequencing.


Attachment: Screenshot of Allsheng Lab Test Data

                                                                    QF                                                                            Fluo-800

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Table 2 Comparison of Inter-well Differences For The Same Concentration of cfDNA


                                                                    QF                                                                            Fluo-800

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Table 3 Comparison of D-value in cfDNA at Different Concentrations

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