Overview

Function Configuration Overview

High Performance and High Throughput
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Feyond-S series adopts a high-speed grating drive mechanism, enabling a full 1 nm-step absorbance spectral scanning from 190 to 1000 nm within 15 seconds. The optimized absorbance optical path achieves a measurement range of 0-4.5 OD, while maintaining a linear correlation coefficient exceeding 0.999 within the 0-4.0 OD range. In addition to conventional absorbance applications, it can detect 16 / 96 micro-volume DNA, RNA, or protein samples simultaneously with the uNano ultra-micro plates.
Flexibility and Sensitivity
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Fluorescence adopts a combined optical path design of monochromators and filters. Users can select either monochromator or filter detection at the excitation and emission ends according to the experimental requirements. This innovative optical path ensures both experimental flexibility and high sensitivity. The fluorescence monochromator adopts adjustable wide / narrow bandwidths with multiple dichroic mirrors to optimize sensitivity and specificity.
Position and Performance
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For fluorescence detection, the instrument adds a Z-axis autofocusing function. It can precisely adjust the the measuring height of the detector based on the liquid level in the well plate, thereby achieving more ideal detection performance.
Luminescence and Spectrum
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Luminescence detection supports both dual luciferase glow kit detection and flash kit detection with an automatic injector. The multicolor luminescence detection module meets diverse luminescence detection needs. Its high-sensitivity luminescence spectral scanning function displays significant application value across multiple fields including food safety, medical diagnostics, and biological detection.
Top Reading and Bottom Reading
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Fluorescence is equipped with both top reading and bottom reading functions. The bottom reading mode more accurately reflects signal changes at the bottom of microplates, helping to reduce errors caused by solution unevenness or impurities. It is more suitable for high-precision experiments such as drug screening and gene expression analysis.
High Temperature and Incubation
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The optimized incubation chamber structure improves incubation performance with precise temperature control up to 66 °C. It supports temperature gradient settings to effectively prevent agglutination. Combined with multiple shaking modes and freely selectable shaking speeds (rpm), it creates a reliable environment for bacterial liquid and enzyme analysis workflows.

Key Configuration

MSS-2 Automatic Injector Module
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Equipped with dual automatic injector modules of the instrument is critically important for a myriad of assays, most notably flash luminescence and Ca²⁺ flux assays etc. The instrument is equipped with a standard injector module, which can meet the precise sample addition operation of 384-well plates and realize the possibility of rapid detection of high-throughput plates.
The automatic injector module can be purchased separated with the device and can also be upgraded later.
Liquid injection protection: The software has a liquid injection protection function to effectively prevent the risk of sample overflow.
ABS Optical Performance Validation Plate
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ABS optical performance validation plate is mainly used to comprehensively evaluate the performance of absorbance function. It can conveniently, quickly and easily check whether absorbance function of the instrument is working properly. It is suitable for the system check of installation and operation qualification.
u-Nano Ultra-Micro Plate
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Quickly complete high-throughput quantification of nucleic acid and proteins without samples dilution.
Independent lower computer software, can quickly read the sample concentration and purity report.
16 / 96 samples can be detected at the same time, only 2~4 μL sample volume is needed.
Filter and Filter Strip
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The easy-to-disassemble modular filter will bring an economical and highly sensitive solution to your fluorescence detection.
Lum-Check Standardization Light Microplate
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Lum-Check standardization light microplate can rapidly check and verify the luminescence function of instruments, ensuring reliable performance.
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User Authority Classification Liberated Process Built-in Algorithms Open Kinetics Kinetics Protection 3D Scanning
  • 1
    User Authority Classification
    Three levels, with independent accounts and passwords.
  • 2
    Liberated Process
    Freely combine detection types and functions according to experimental needs.
  • 3
    Built-in Algorithms
    Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis.
  • 4
    Open Kinetics
    During kinetics detection, the process can be paused and resumed, allowing other detection experiments to be inserted to enhance detection efficiency.
  • 5
    Kinetics Protection
    During longtime kinetics detections, if the computer occurs an abnormal disconnection, the instrument will automatically save the detection data. After reconnection, the data will be uploaded.
  • 6
    3D Scanning
    Simultaneous excitation and emission wavelength scanning for direct observation of sample features.
Model

Feyond-S300 Pro

Feyond-S400

Feyond-S400 Pro

ABS-grating
FL-grating
FL-filter

FRET
LUM-grating

BRET-filter


TRF



TR-FRET-filter




Model
Feyond-S300
Technical Parameter
ABS
Light sourceHigh-energy xenon lamp
DetectorPD×2
Wavelength selection (monochromator)Dual-grating
Wavelength accuracy2 nm
Wavelength repeatability (SD)±1 nm
Half width (FWHM)≤5 nm
Wavelength range190~1000 nm, 1 nm step
Measure range0~4.5 OD
Resolution0.0001 OD
Accuracy96-precision mode: ±(1.0%+0.003 OD) @(0.0-2.0 OD]; ±2.0% @(2.0-3.0 OD]
RepeatabilityCV <1.0% fast (0.0 - 3.0 OD]; CV <0.5% accurate (0.0 - 3.0 OD]
Stray light<0.005% @230 nm
LinearR2 ≥0.999 @[0.0 - 4.0 OD) @230 nm
Reading time

96-well plate: endpoint: fast <15s (A1 to H1)

Spectral scanning: fast <15s (190~1000 nm, 1 nm step)

Optical path correction technologyWith optical pathlength correction function
FL
Excitation light sourceHigh-energy xenon lamp
Light source energyCan be adjusted based on sample signal intensity, with low and high energy intensity options available
DetectorPMT
Reading modeTop reading, bottom reading
Wavelength rangeEX: 250~830 nm; EM: 270~850 nm
Half width15 nm, 25 nm
Wavelength accuracy (grating)EX <2 nm; EM <2 nm
Wavelength repeatability (grating)<1 nm
Linear dynamic range6 logs
Z-axis adjustmentWith Z-axis height automatic adjustment (top / bottom reading), suitable for consumables of different heights and reagents of different volumes
Wavelength selectionFour-grating monochromator
Detection limit

Grating:

≤2.5 pM (1 nM fluorescein sodium) top reading

≤25 pM (1 nM fluorescein sodium) bottom reading

LUM
DetectorPMT
Wavelength range300~700 nm
Detection limit≤35 amol/well flash ATP
Linear dynamic range6 logs
Crosstalk≤0.05% (ATP @96-well white plate)
FilterLUM
Basic Parameter
FunctionAbsorbance (ABS), fluorescence (FL, FRET), luminescence (Glow, Flash, BRET)
Read modeEndpoint, kinetics, spectral scanning, and well scanning (higher than 30×30 density/well)
Plate6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, 16 / 96 uNano ultra-micro plate
Optical path featuresHigh-precision four-grating optical path
Software
Software installationChinese / English, Win 10 and above, 64-bit
Modular, streamlinedModular, streamlined functional operations with freely adjustable program editing steps
Calculation & editingMultiple calculations on raw data to create standard curves and sample detection data
Version configurationResearch version (standard), complying with 21 CFR Part 11 regulatory certification requirements (optional)
Others
Automation compatibilityCompatible with microplate stacks, can continuously read 20 plates; compatible with Allsheng automated instruments; third-party automated instruments (optional)
Instrument port1 USB B port; RS232 interface
Dimension (W×D×H)420×550×385 mm
Power supplyAC 100~240 V, 50~60 Hz
Power100~240 V wide voltage 2 A
Weight45 kg
Accessory Parameter
Automatic Injector
Plate6-well, 12-well, 24-well, 48-well, 96-well, 384-well
Quantity2
Dispensing volume5~1000 μL, 1 μL increment
Liquid injection speed125~500 μL/s
Accuracy±1 μL @5~50 μL; ±2% @51~1000 μL
Waste liquid collection25 mL
Shaking & Incubation
Shaking modeLinear, circular, double circular
Shaking amplitude1 mm~6 mm
Shaking frequencySlow, medium, fast (4~19 Hz)
Incubation temp.RT. +5 °C~66 °C (can be preheated)
Temp. control precision≤0.25 °C @37 °C
Temp. uniformity1 °C @37 °C


CodeProduct description
AS-19210-00Feyond-S300 microplate reader (multi-mode)
AS-19211-01ReaderIt-A1 PC analysis software
AS-19211-02485 nm / 530 nm (fluorescence standard)
AS-19211-03523 nm / 564 nm (fluorescence standard)
AS-19211-05LUM cut-off filter (standard)
AS-19091-01Lum-check standardization light microplate
AS-19011-02u-Nano 16 ultra-micro plate
AS-19011-03ABS optical performance validation plate
AS-19011-04MSS-2 automatic injector
AS-19011-05u-Nano 96 ultra-micro plate


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