Automated NGS Library Preparation Workstation

Auto-NGS 180S

A full-featured and compact automated NGS library preparation workstation can meet the needs of library preparation, quantitation, multiple targeting and hybrid capture only one time. The instrument is equipped with a 24-well fluorometer and PCR amplification block, truly achieving convenient operation of sample-to-library, without manual intervention throughout the whole process.
The design of 22 standard plates of the instrument, combined with grippers and plate stacking function, can meet the relatively simple library preparation requirements of 96 samples, as well as the fully automated library preparation and hybrid capture requirements of 16 sample simplification types.

INQUIRY

Mid-to-high throughput platform, supporting the whole process automated library preparation
Open platform, suitable for most of the library preparation cartridges on the market
Smart and visual software, clear logic and easy to use

Technical Specifications

Ordering Information

Overview

Key Configuration

Plate Layout

24-Channel Pipettor

Pipetting range 1~200 μL, 24-channel fixed spacing pipettor can be used as 24 channels, 16 channels, 8 channels, single channel or any channel.

Fluorometer

The fluorometer block, independently developed and designed by Allsheng, meets concentration measurement requirements during library preparation. With software, it can realize automated normalization, pooling, etc.

24-well design, can test 24 samples simultaneously.

Lowest detection limit down to 0.02 ng/μL (dsDNA).

Accurate quantitation and high accuracy with only 2-20 μL samples.

PCR Amplification Block

Block temperature control range: 4 °C ~ 99 °C, the temperature range of the thermo lid is 30 °C~120 °C

Temperature precision: ≤0.2 °C @55 °C, temperature accuracy ≤±0.3 °C @55 °C

Temperature uniformity: ≤0.7 °C (@55 °C, 72 °C)

Thermo Shaker Incubator Block

A thermo shaker incubator applied to Allsheng automation workstation. The incubator can be used for heating, incubation and mixing of deepwell plates. The high-precision zero positioning function ensures the safe pipetting by the pipettor, with the anti-vibration technology and 2D motion control.

Temperature range: 30 °C~105 °C

Amplitude: 2 mm, max speed: 3000 rpm

Temperature Control Block

Can be freely set at 4~105 °C. It's the standard temperature control block of NGS series. According to the usages, bock adapters can be customized to meet different consumables requirements.

Temperature accuracy: ≤1 °C

Temperature uniformity: ≤1 °C, @55 °C

1

Simple Reagent Input

Free selection of well positions, and support for pre-filled reagents
2

Clear Software Logic

The cartridge running framework is completed through drag and drop operations
3

Detailed Operation Guidance

Follow the software prompts to place consumables in order to run the program
4

Graphical Step Editing

Easy to get started, with built-in multiple liquid parameters matching precise pipetting requirements

Model	Auto-NGS 100R	Auto-NGS 100RF	Auto-NGS 180S	Auto-NGS 200
Throughput	24	24	48	96
Pipettor	8-channel	8-channel	24-channel	24-channel
Pipetting range	1-200 μL	1-200 μL	1-200 μL	1-200 μL
Shaking block	/	/	√	√
Quality control block	/	√	√	√
PCR block	√	√	√	√
Dimension (W×D×H)	799×767×775 mm	800×774×775 mm	980×793×830 mm	1420×790×800 mm

Note: 48 refers to the instrument's maximum throughput

Model	Auto-NGS 180S
Throughput	8~24
Plate number	Up to 9 tip boxes, including 5 single-layer tip tiles and 2 double-layer tip tiles
Pipettor	200 μL; 24-channel (can be used as a single channel)
Pipettor	Minimum detection volume: 20 μL
Pipetting range	1~200 μL
Pipetting precision (CV)	1 μL≤8 %; 2 μL≤5 %; 5 μL≤4 %; 20 μL≤1 %; 100 μL≤1 %; 200 μL≤1 %
Pipetting accuracy (A)	1 μL≤±10 %; 2 μL≤±8 %; 5 μL≤±4 %; 20 μL≤±2 %; 100 μL≤±1 %; 200 μL≤±1 %
Cooling block	2 cooling blocks, can be used to save cold-storage reagents
Cooling block	Temperature control range: 4~105 °C
Cooling block	Temperature control accuracy: ≤1 °C
Cooling block	Temperature control uniformity: ≤1 °C @55 °C
Heating mixing block	1 heating mixing block
Heating mixing block	Temperature control range: 30 °C~105 °C
Heating mixing block	Temperature control accuracy: ±0.5 °C
Heating mixing block	Temperature uniformity: ≤1 °C @55 °C
Thermal cycling block	Temperature control range: thermo lid: 30 °C~120 °C; block: 4 °C~99 °C
Thermal cycling block	Uniformity: ≤0.7 °C
Thermal cycling block	Accuracy: ≤±0.3 °C
Fluorescence detection	Repeatability: CV ≤1.5 %
Fluorescence detection	Inter-well repeatability: CV ≤10 % (0.4 ng ≤ Total dsDNA＜1 ng)
Fluorescence detection	Inter-well repeatability: CV ≤5 % (1 ng ≤ Total dsDNA＜2 ng)
Fluorescence detection	Inter-well repeatability: CV ≤2 % (Total dsDNA >2 ng)
Fluorescence detection	Accuracy: ≤15 % (0.4 ng, compared to Qubit)
Magnetic plate	96-well annular magnetic plate
Gripper	Yes
Consumable stacking mode	Consumable stack
UV sterilization	Equipped with UV sterilization lamp, HEPA device
Instrument port	USB port
Information management	External scanner for entering sample / reagent information
Information management	Expandable LIS system connection
Information management	Expandable cloud database connection
Operating environment	Temperature requirement: 15 °C~25 °C; Relative humidity: ≤80 %
Power supply	100~240 V, 50 / 60 Hz, rated power 1700 VA
Dimension (W×D×H) mm	1085×795×840 mm