Overview

Function Configuration Overview

Absorbance
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Using the monochromator design, it only need 15 s for continuous spectral scanning in 1 nm increments between 190~1000 nm. It supports ultraviolet to near-infrared spectrum analysis, and the wavelength can be freely selected to easily realize various application detection. In addition, the linear correlation coefficient can reach more than 0.999 within 0-3.5OD.
Fluorescence
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Utilizing a combination of xenon lamps, filters, and high-sensitivity photomultiplier tube (PMT), this system significantly enhances detection sensitivity. The automated filter wheel design supports up to 8 excitation and emission filter combinations, offering 64 customizable configurations without manual module replacement. It enables simultaneous detection of 8 fluorescence channels per cycle, effortlessly achieving both fluorescence intensity and FRET (Fluorescence Resonance Energy Transfer) measurements.
For multi-channel detection applications, the software has two reading modes, which can be selected to read by well or by plate according to experimental needs.
Luminescence
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Utilizing a LUM PMT detector with BRET filters and customized data analysis algorithms, it enables the detection of multicolor luminescence experiments. The 8-position filter wheel design allows both direct measurement and customization of alternative wavelength filters. At the same time, the instrument can be combined with an automatic injector to realize the efficient detection of flash reagent kits
Multiple Shaking Modes
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There are three types of shaking modes: linear, circular, double circular, and a variety of shaking speeds (rpm) can be freely selected, which is more conducive to the realization of kinetic background vibration of different types of samples.
Well Scanning Function
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The scanning detection method of up to 900 points per well is realized by using flexible orbital motion and accurate detection site, which reduces the difference reading caused by different positions.
Optical Pathlength Correction
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Using built-in algorithms, automatically convert absorbance values measured at different solution volumes in the plate to equivalent values at a 1 cm optical pathlength.

Key Configuration

u-Nano Ultra-Micro Plate
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Quickly complete high-throughput quantification of nucleic acid and proteins without samples dilution.
Independent lower computer software, can quickly read the sample concentration and purity report.
16 / 96 samples can be detected at the same time, only 2~4 μL sample volume is needed.
ABS Optical Performance Validation Plate
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ABS optical performance validation plate is mainly used to comprehensively evaluate the performance of absorbance function. It can conveniently, quickly and easily check whether absorbance function of the instrument is working properly. It is suitable for the system check of installation and operation qualification.
MSS-2 Automatic Injector Module
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Equipped with dual automatic injector modules of the instrument is critically important for a myriad of assays, most notably flash luminescence and Ca²⁺ flux assays etc. The instrument is equipped with a standard injector module, which can meet the precise sample addition operation of 384-well plates and realize the possibility of rapid detection of high-throughput plates.
The automatic injector module can be purchased separated with the device and can also be upgraded later.
Liquid injection protection: The software has a liquid injection protection function to effectively prevent the risk of sample overflow.
Lum-Check Standardization Light Microplate
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Lum-Check standardization light microplate can rapidly check and verify the luminescence function of instruments, ensuring reliable performance.
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Built-In Intelligent Touch Screen Version PC Control Version: User Authority Classification Liberated Process Built-in Algorithms Open Kinetics Kinetics Protection 3D Scanning
  • 1
    Built-In Intelligent Touch Screen Version
    The standard 10-inch rotating touch screen is equipped with built-in software to independently complete parameter setting and data processing; the rotation angle of the screen is ≤90°, and the angle can be manually adjusted to adapt to researchers of different heights.
  • 2
    PC Control Version: User Authority Classification
    Three levels, with independent accounts and passwords.
  • 3
    Liberated Process
    Freely combine detection types and functions according to experimental needs.
  • 4
    Built-in Algorithms
    Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis.
  • 5
    Open Kinetics
    During kinetics detection, the process can be paused and resumed, allowing other detection experiments to be inserted to enhance detection efficiency.
  • 6
    Kinetics Protection
    During longtime kinetics detections, if the computer occurs an abnormal disconnection, the instrument will automatically save the detection data. After reconnection, the data will be uploaded.
  • 7
    3D Scanning
    Simultaneous excitation and emission wavelength scanning for direct observation of sample features.
Model
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Feyond-MF200

(2-Mode)

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Feyond-ML200

(2-Mode)

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Feyond-M300

(3-Mode)

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Feyond-M400

(4-Mode)

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Feyond-M500

(5-Mode)

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Feyond-M300_Pro
(3-Mode)

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ABS
FL

FRET
LUM

BRET





TRF




TR-FRET






FP





Incubation





ModelFeyond-M300
ABS
Plate6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, 16 / 96 uNano ultra-micro plate
Reading time (spectrum)190~1000 nm <15s (1 nm step)
Light sourceXenon lamp
Wavelength accuracy2 nm
Wavelength repeatability(SD)0.2 nm
Half width (FWHM)<2.5 nm
DetectorPD×2
Wavelength range190~1000 nm, 1 nm step
Measure range0 - 4 OD
Resolution0.0001 OD
Accuracy @450 nmPrecision mode: ±(1.0%+0.003OD) @(0.0-2.0OD]; ±2%>@(2.0-3.0OD]
Repeatability @450 nmCV<1.0% or SD<0.003 fast (0.0-3.0OD]; CV<0.5% or SD<0.003 accurate (0.0-3.0OD]
Stray light0.1% @220 nm
LinearR²≥0.999 @[0.0 - 3.0 OD]
Reading time96-well plate: fast <15s (A1 to H1)
FL
Reading modeTop reading
Excitation light sourceXenon lamp
DetectorPMT
Wavelength rangeEX: 200~1000 nm; EM: 270~850 nm
Filter EX / EM3 groups: EX470 / EM525, EX523 / EM564, EX624 / EM692 (other wavelengths can be replaced)
Linear dynamic range≥6 logs
Detection limit2 pM (optimization condition)
LUM
DetectorPMT
Detection limit≤25 amol/well @96-well white plate with flash ATP
Linear dynamic range≥6 logs
Crosstalk≤ 0.005%
Wavelength range200~850 nm
FilterLUM
Software
Analysis softwareReaderIt-S1 software
Basic Parameter
Read typeEndpoint, kinetics, spectral scanning, spectral kinetics, and well scanning
Plate6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, 16 / 96 uNano ultra-micro plate
Accessories16 / 96 uNano ultra-micro plate, auto-injector
Shaking modeLinear, circular, double circular (strength and speed adjustable)
Incubation temperatureRT. +4 °C ~ 45 °C
Temperature accuracy±0.5 °C @37 °C
Software interfaceChinese / English
Screen size10-inch
Operation methodTouch screen (Android system) / PC (Windows system)
Data capacity10 GB
CompatibilitySupport PC software, Win 10 and above, 64 bit
Network transmissionThe test data report can be uploaded to the PC server through FTP
Instrument port

Touch screen version: 2 USB A ports, 1 USB B port, 1 Ethernet port, Rs232 bus interface

PC control version: 1 USB B port, Rs232 bus interface

Power supplyAC 100-240 V, 50-60 Hz
Size (W×D×H)320×435×305 mm
Weight25 kg
Accessory Parameter
u-Nano
Sample numberu-Nano 16: 1~16, u-Nano 96: 1~96
Sample detection volume2-4 μL
Automatic Injector
Support plate6-384 well plate
Quantity1 / 2
Dispensing volume5-1000 μL, 1 μL increment
Liquid injection speed125~500 μL/s
Accuracy±1 μL @ 5-50 μL; ±2% @ 51-1000 μL
Waste liquid collection25 mL


CodeProduct description
AS-19150-00Feyond-M300 microplate reader
AS-19111-01ReaderIt-S1 PC analysis software
AS-19111-02470nm-525nm fluorescence filter (standard)
AS-19111-03523nm-564nm fluorescence filter (standard)
AS-19111-04624nm-692nm fluorescence filter (standard) (M300/M380/M300_Pro/M380_Pro/M400/M480/M400_Pro/M480_Pro/M500/M580/M500_Plus/M580_Plus)
AS-19111-07LUM cut-off filter
AS-19011-02u-Nano 16 ultra-micro plate
AS-19011-03ABS optical performance validation plate
AS-19011-04MSS-2 automatic injector
AS-19011-05u-Nano 96 ultra-micro plate
AS-19091-01Lum-check standardization light microplate


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