Microplate Reader
Feyond-MF200
Feyond-MF200, as a distinctive dual-function microplate detection platform, adopts a unique design of dual optical elements. It features two detection modes: full-wavelength absorbance (UV / Vis / NIR) and fluorescence intensity (FI).
The absorbance detection mode covers a wide wavelength range from ultraviolet to visible light, enabling precise measurement of the degree to which the sample absorbs light at different wavelengths.
The fluorescence detection mode, on the other hand, makes use of the characteristic that fluorescent substances emit fluorescence under the excitation of light at a specific wavelength. It has high sensitivity and specificity, and is suitable for detecting trace amounts of biomolecules, intracellular signal transduction, analysis of enzyme activity, etc., playing an important role in fields such as drug screening and cell biology research.
The absorbance detection mode covers a wide wavelength range from ultraviolet to visible light, enabling precise measurement of the degree to which the sample absorbs light at different wavelengths.
The fluorescence detection mode, on the other hand, makes use of the characteristic that fluorescent substances emit fluorescence under the excitation of light at a specific wavelength. It has high sensitivity and specificity, and is suitable for detecting trace amounts of biomolecules, intracellular signal transduction, analysis of enzyme activity, etc., playing an important role in fields such as drug screening and cell biology research.
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Absorbance wavelength range: 190–1000 nm -
Support up to 8 excitation and emission filter combinations, easily achieving FRET measurements -
Fluorescence detection limit as low as 2 pM
Overview
Absorbance
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Using the monochromator design, it only need 15 s for continuous spectral scanning in 1 nm increments between 190~1000 nm. It supports ultraviolet to near-infrared spectrum analysis, and the wavelength can be freely selected to easily realize various application detection. In addition, the linear correlation coefficient can reach more than 0.999 within 0-3.5OD.
Fluorescence
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Utilizing a combination of xenon lamps, filters, and high-sensitivity photomultiplier tube (PMT), this system significantly enhances detection sensitivity. The automated filter wheel design supports up to 8 excitation and emission filter combinations, offering 64 customizable configurations without manual module replacement. It enables simultaneous detection of 8 fluorescence channels per cycle, effortlessly achieving both fluorescence intensity and FRET (Fluorescence Resonance Energy Transfer) measurements.For multi-channel detection applications, the software has two reading modes, which can be selected to read by well or by plate according to experimental needs.
Multiple Shaking Modes
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There are three types of shaking modes: linear, circular, double circular, and a variety of shaking speeds (rpm) can be freely selected, which is more conducive to the realization of kinetic background vibration of different types of samples.
Well Scanning Function
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The scanning detection method of up to 900 points per well is realized by using flexible orbital motion and accurate detection site, which reduces the difference reading caused by different positions.
Key Configuration
u-Nano Ultra-Micro Plate
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Quickly complete high-throughput quantification of nucleic acid and proteins without samples dilution.Independent lower computer software, can quickly read the sample concentration and purity report.16 / 96 samples can be detected at the same time, only 2~4 μL sample volume is needed.
ABS Optical Performance Validation Plate
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ABS optical performance validation plate is mainly used to comprehensively evaluate the performance of absorbance function. It can conveniently, quickly and easily check whether absorbance function of the instrument is working properly. It is suitable for the system check of installation and operation qualification.
MSS-2 Automatic Injector Module
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Equipped with dual automatic injector modules of the instrument is critically important for a myriad of assays, most notably flash luminescence and Ca²⁺ flux assays etc. The instrument is equipped with a standard injector module, which can meet the precise sample addition operation of 384-well plates and realize the possibility of rapid detection of high-throughput plates.The automatic injector module can be purchased separated with the device and can also be upgraded later.Liquid injection protection: The software has a liquid injection protection function to effectively prevent the risk of sample overflow.
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1Built-In Intelligent Touch Screen VersionThe standard 10-inch rotating touch screen is equipped with built-in software to independently complete parameter setting and data processing; the rotation angle of the screen is ≤90°, and the angle can be manually adjusted to adapt to researchers of different heights.
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2PC Control VersionIt is suitable for traditional operation habits, and realizes computer control and high-throughput data analysis through ReaderIt-S1 software.
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Model | |||||||
ABS | ● | ● | ● | ● | ● | ||
FL | ● | ● | ● | ● | ● | ||
LUM | ● | ● | ● | ● | ● | ||
TRF | ● | ● | |||||
FP | ● | ||||||
Incubation | ● | ● | ● | ● | ● | ● | ● |
| Model | Feyond-MF200 |
| ABS | |
| Plate | 6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, uNano 16 / 96 ultra-micro plate |
| Reading time (spectrum) | 190-1000 nm <15s (1nm step) |
| Light source | Xenon lamp |
| Wavelength accuracy | 2 nm |
| Wavelength repeatability(SD) | 0.2 nm |
| Half width (FWHM) | <2.5 nm |
| Detector | PD×2 |
| Wavelength range | 190~1000 nm, 1 nm step |
| Measure range | 0-4 OD |
| Resolution | 0.0001 OD |
| Accuracy @450 nm | Precision mode: ±(1.0%+0.003OD) @ (0.0-2.0OD]; ±2%>@(2.0-3.0OD] |
| Repeatability @450 nm | CV<1.0% or SD<0.003 fast (0.0-3.0OD]; CV<0.5% or SD<0.003 accurate (0.0-3.0OD] |
| Stray light | 0.1% @220 nm |
| Linear @450 nm | R²≥ 0.999 @ [0.0 - 3.0 OD] |
| Reading time | 96-well plate: fast <15 s (A1 to H1) |
| FL | |
| Reading mode | Top reading |
| Excitation light source | Xenon lamp |
| Detector | PMT |
| Wavelength range | EX: 200 - 1000 nm; EM: 270 - 850 nm |
| Filter EX / EM | 3 groups: EX470 / EM525, EX523 / EM564, EX624 / EM692 (other wavelengths can be replaced) |
| Linear dynamic range | ≥6 logs |
| Detection limit | 2 pM (optimization condition) |
| Software | |
| Analysis software | ReaderIt-S1 software |
| Basic Parameter | |
| Read type | Endpoint, kinetics, spectral scanning, spectral kinetics, and well scanning |
| Plate | 6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, uNano 16 / 96 ultra-micro plate |
| Accessories | u-Nano16 plate, u-Nano96 plate, auto-injector |
| Shaking mode | Linear, circular, double circular (strength and speed adjustable) |
| Incubation temperature | RT. +4 °C ~ 45 °C |
| Temperature accuracy | ±0.5 °C @37 °C |
| Software interface | Chinese / English |
| Screen size | 10-inch |
| Operation method | Touch screen (Android system) / PC (Windows system) |
| Data capacity | 10 GB |
| Compatibility | Support PC software, Win 10 and above, 64 bit |
| Network transmission | The test data report can be uploaded to the PC server through FTP |
| Instrument interface | Touch screen version: 2 USB A ports, 1 USB B port, 1 Ethernet port, Rs232 bus interface PC control version: 1 USB B port, Rs232 bus interfac |
| Power supply | AC 100-240 V, 50-60 Hz |
| Dimension (W×D×H) | 320×435×305 mm |
| Weight | 25 kg |
| Code | Product description |
| AS-19110-00 | Feyond-MF200 Microplate Reader |
| AS-19111-01 | ReaderIt-S1 PC analysis software |
| AS-19111-02 | 470nm-525nm fluorescence filter (standard) |
| AS-19111-03 | 523nm-564nm fluorescence filter (standard) |
| AS-19111-04 | 624nm-692nm fluorescence filter (M300_Pro/M400_Pro/M500_Plus as standard) |
| AS-19011-02 | u-Nano16 ultra-micro plate |
| AS-19011-03 | ABS optical performance validation plate |
| AS-19011-04 | MSS-2 automatic injector |
| AS-19011-05 | u-Nano96 ultra-micro plate |
Product Information
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