Sample Lysis & Grinding
Due to the fact that multiple bacterial species are often mixed together in metagenomic sequencing samples, many samples are difficult to break through, such as Mycobacterium tuberculosis and Cryptococcus in respiratory samples. Using the homogenizer combined with different grinding media can effectively ensure the detection of difficult broken pathogens, while optimizing protocols to preserve the integrity of nucleic acids from easily broken pathogens.
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Nucleic Acid Extraction
When the detection method for pathogenic microorganisms is amplicon sequencing technology or hybrid sequencing technology, samples often are required of PCR amplification and library preparation before subsequent detection. The initial sample concentration requirement is not very high, and the 1 mL reaction system of Auto-Pure 32A / 96 nucleic acid purification system can meet the demand. Open software design that can adapt to various extraction cartridges on the market. Users can choose the appropriate models for extraction based on their actual needs such as reaction system and maximum sample size per run.
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